Introduction.Fred Blattner Lab conducted a set of gene-expression microarray experiments on Escherichia coli K12 to monitor changes in transcription abundance in response to a heat shock treatment reported here. Experiments were done with hibridization of radiolabeled cDNA to spot blots on nylon (radio-hs), and with hibridization of fluorecently-labeled cDNA to glass microaarys (micro-hs).
The cells used in the experiments were grown in LB defined media (Luria-Bertini broth) to an OD A600 between 0.8 and 0.9 at 37oC. Heat shock induction was carried to 50oC. For purpose of our analysis here we use the ratios that are published in the paper,
Craig S. Richmond, Jeremy D. Glasner, Robert Mau, Hongfan Jin, and Frederick R. Blattner (1999) Genome-wide expression profiling in Escherichia coli K-12. Nucleic Acids Research 27(19), 3821-3835. (Download pdf file).In this page we present an analysis of the radio-hs experiment using the GETools web tool to analyse the expression profile data. The results of this analysis are showed in the following pages:
- Regulatory Data to HS-response experiment at 5 fold.
- Summary Table of HS-response experiment at 5 fold.
- ViewGraph of affected TU's at 5 fold.
- Detection of new putative biding sites corregulating genes.
- Multiple Gene Analysis:
Affected TU's in radio & micro HS experiments at 5 fold.
